Know more

About cookies

What is a "cookie"?

A "cookie" is a piece of information, usually small and identified by a name, which may be sent to your browser by a website you are visiting. Your web browser will store it for a period of time, and send it back to the web server each time you log on again.

Different types of cookies are placed on the sites:

  • Cookies strictly necessary for the proper functioning of the site
  • Cookies deposited by third party sites to improve the interactivity of the site, to collect statistics

Learn more about cookies and how they work

The different types of cookies used on this site

Cookies strictly necessary for the site to function

These cookies allow the main services of the site to function optimally. You can technically block them using your browser settings but your experience on the site may be degraded.

Furthermore, you have the possibility of opposing the use of audience measurement tracers strictly necessary for the functioning and current administration of the website in the cookie management window accessible via the link located in the footer of the site.

Technical cookies

Name of the cookie


Shelf life

CAS and PHP session cookies

Login credentials, session security



Saving your cookie consent choices

12 months

Audience measurement cookies (AT Internet)

Name of the cookie


Shelf life


Trace the visitor's route in order to establish visit statistics.

13 months


Store the anonymous ID of the visitor who starts the first time he visits the site

13 months


Identify the numbers (unique identifiers of a site) seen by the visitor and store the visitor's identifiers.

13 months

About the AT Internet audience measurement tool :

AT Internet's audience measurement tool Analytics is deployed on this site in order to obtain information on visitors' navigation and to improve its use.

The French data protection authority (CNIL) has granted an exemption to AT Internet's Web Analytics cookie. This tool is thus exempt from the collection of the Internet user's consent with regard to the deposit of analytics cookies. However, you can refuse the deposit of these cookies via the cookie management panel.

Good to know:

  • The data collected are not cross-checked with other processing operations
  • The deposited cookie is only used to produce anonymous statistics
  • The cookie does not allow the user's navigation on other sites to be tracked.

Third party cookies to improve the interactivity of the site

This site relies on certain services provided by third parties which allow :

  • to offer interactive content;
  • improve usability and facilitate the sharing of content on social networks;
  • view videos and animated presentations directly on our website;
  • protect form entries from robots;
  • monitor the performance of the site.

These third parties will collect and use your browsing data for their own purposes.

How to accept or reject cookies

When you start browsing an eZpublish site, the appearance of the "cookies" banner allows you to accept or refuse all the cookies we use. This banner will be displayed as long as you have not made a choice, even if you are browsing on another page of the site.

You can change your choices at any time by clicking on the "Cookie Management" link.

You can manage these cookies in your browser. Here are the procedures to follow: Firefox; Chrome; Explorer; Safari; Opera

For more information about the cookies we use, you can contact INRAE's Data Protection Officer by email at or by post at :


24, chemin de Borde Rouge -Auzeville - CS52627 31326 Castanet Tolosan cedex - France

Last update: May 2021

Menu Logo Principal INRAE Université de Liège Université de Lille Université Picardie Jules Verne Associated institutions

UMR Transfrontalière BioEcoAgro (

Thesis Chassiclip

Chassiclip: Creation of bacterial chassis strains for the heterologous production of lipopeptides and optimization of production, extraction and purification processes

ESTABLISHMENT: University of Lille

Laboratory of affiliation: UMRt 1158 BioEcoAgro

Scientific field, Speciality: DS10 | Food Biotechnology, Food Science, Physiology


Thesis director: LECLERE Valérie, Pr,

Co-supervisor:  DUBAN Matthieu, IgR,

Affiliate programme(s): ex. labex, ERC, Horizon Europe, etc

Planned (co)-funding (mention: in progress/obtained): Région HdF, ED SMRE


Title of the thesis:

Chassiclip: Creation of bacterial chassis strains for the heterologous production of lipopeptides and optimization of production, extraction and purification processes


Cyclic lipopeptides or CLiPs (Cyclic LipoPeptides) are microbial secondary metabolites with surfactant and antifungal properties that make it possible to consider their use in biocontrol applications. They can act through direct antagonism mechanisms towards the pathogen (biocides) or indirectly by inducing the host plant's systemic defence response (SDP) (Ongena and Jacques, 2008).

CLiPs are synthesised by different bacterial genera (notably Bacillus, Pseudomonas or Burkholderia) through non-ribosomal synthesis pathways, thanks to modular mega-enzymes called non-ribosomal peptide synthetases or NRPSs (Marahiel, 2016). NRPSs work as production chains to catalyse, step by step, the assembly of amino acids leading to the construction of non-ribosomal peptides. NRPS are encoded by very large genes, gathered in biosynthetic gene clusters (BGCs) of several tens of thousands of bases, which also contain non-NRPS genes essential for the synthesis of non-ribosomal peptides. Bioinformatics tools are now available to detect the presence of such BGCs in genomes and to predict the synthesis of CLiPs in silico (Pupin et al., 2018).

Several bottlenecks to the use of CLiPs in biocontrol have been identified. CLiPs are generally produced naturally in small quantities (at most a few tens of mg per litre of culture), and a single strain is often capable of co-producing several lipopeptides in a mixture (D'aes et al., 2014), which poses difficulties in terms of their extraction/purification. On the other hand, some BGCs are even cryptic, i.e. not expressed under laboratory culture conditions. One way of getting around these difficulties is therefore to use heterologous production, which consists of using a host strain into which BGCs containing biosynthesis genes are introduced. The proposed subject involves the molecular construction of chassis strains by genetic engineering, capable of receiving lipopeptide BGCs of various origins. Optimisation of the production, extraction and purification processes is envisaged from one or two models in order to obtain sufficient quantities to analyse their structure, understand their mode of action, and evaluate their activities at different scales (laboratory, greenhouse, field).


Expected date of recruitment: 1st October 2023

Expected skills: microbiology, molecular biology, fermentation