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INRAE

24, chemin de Borde Rouge -Auzeville - CS52627 31326 Castanet Tolosan cedex - France

Last update: May 2021

Menu Logo Principal INRAE Université de Liège Université de Lille Université Picardie Jules Verne Associated institutions

UMR Transfrontalière BioEcoAgro (www.bioecoagro.eu)

Thesis - The effect of chicory microbiota on plant composition

ESTABLISHMENT: University of Lille

Laboratory(ies) of affiliation: BioEcoAgro UMR 1158

Scientific field, Speciality: DS5 | Molecular and Cellular Aspects of Biology

Thesis director: Lucau-Danila Anca, MCF anca.lucau@univ-lille.fr

Co-director: Hilbert Jean-Louis Prof, jean-louis.hilbert@univ-lille.fr

Drider Djamel, Prof, djamel.drider@univ-lille.fr

Siah Ali, Prof, ali.siah@junia.com

Planned (co)-funding: funds (50%) obtained, regional research allocations 2023 (50%) in progress

Title of the thesis: The effect of chicory microbiota on plant composition

THESIS SUBJECT
Industrial chicory (Cichorium intybus var. sativum) is recognized as a functional food and different classes of molecules present in the plant have been identified as responsible for the beneficial effects on animal and human health (Fouré et al., 2018; Pouille et al. , 2020; Pouille et al., 2022). The chemical composition of chicory and its various foodstuffs can vary depending on the genotype and growing conditions. The microbial ecosystem associated with the plant is also likely to influence its composition.
The objective of the project is to identify and quantify the microorganisms associated with industrial chicory and to find a link with its phytochemical composition. For this, the project aims to analyze the microbiota of the rhizosphere, the endophyte microbiota of the root and also that associated with chicory seeds, by targeted sequencing methods (16S and ITS) in order to establish a link with the chemical composition of the plant. This composition will be monitored via metabolomic analyses. Several experimental devices will be set up to evaluate the variability due to the station (properties and mineralogical composition of the soil, water stress conditions, etc.) and to the genotype (several varieties and genotypes will thus be tested at different stages of plant development). Following the mapping of bacterial and fungal taxa, cultivable microorganisms that are of interest for the production of bioactive substances, for the extraction of phosphates, for plant defense, growth and resistance to drought, will be isolated and characterized to offer solutions for biocontrol and improvement of the food quality of chicory.

Expected date of recruitment: 01/10/ 2023

Contact: anca.lucau@univ-lille.fr

Candidate profile:
- hold an MSc in molecular biology, biochemistry or other related disciplines
- have knowledge of omics analysis
- be motivated and passionate about research
- have a good academic record and good writing skills and scientific rigor
- be diligent in their work
- have a good ability to communicate and work in a team

Deadline for application: 01/05/2023