BioEcoAgro Joint Cross-Border Research Unit

Food and biochemical pilot plan - ULille

Food and biochemical pilot plan

Bioactivity technical platform: development and use of techniques for evaluating the biological activities of peptides

Cell culture room, Animal house, Microplate reader, Radioactive room

ICV autres equipements
© Université de Lille

The objective of this platform is to set up assay techniques but also methodologies for understanding the mechanisms of action of peptides. In order to meet the needs of the different teams in the laboratory and the demands of industry, the screening of certain biological activities, In order to meet the needs of the different teams in the laboratory and the demands of industry, the screening of certain biological activities, in particular opioid (involvement in the regulation of stress and food intake), ACE (Angiotensin Converting Enzyme) inhibitor (antihypertensive), regulator of the secretion of intestinal hormones (CCK/GLP-1 and DPP-IV), is carried out by simple in vitro assays and cellular models, anti-inflammatory and anti-oxidant. In vivo assays are performed on hydrolysates (or hydrolysate fractions) that are particularly active in vitro to characterise and confirm the biological activity of interest. The cytotoxicity of fractions or extracts is also evaluated and constitutes a strong expertise of the technical platform.
    A close link between in vitro and in vivo tests will allow confirmation of the supposed biological mechanisms.

Other facilities of excellence:

    Bioreactors
    • Ambr15 Minibioreeactor
    • 3 MiniBio Fermenters
    • Fully Regulated Enzyme Bioreactors
    • 5/10L Fermeters + Peristaltic Pump
    • Liquid-Solid pump

    Biochemistry/Molecular Biology
    • UV/Vis/Fluorimetry/Luminometry spectrophotometer
    • 2 Flux Cytometers
    • PCR Thermocyclers

    Samples Treatment
    • Atomiser
    • Cells Grinder
    • Ultracentrifuge
    • Dessicator
    • Cryostat
    • Microplates Speedvac
    • Lyophilisator
    • 3L Rotavapor
    • 20L Industrial Rotovapor

    Analytical Systems
    • HR Qtof Mass Spectrometer
    • MS Puriflash system + TLC-MS
    • HPLC DAD - Corona
    • Automated Migration Cuve
    • Automated TLC visualization system
    • HPTLC Vario System
    • Ionic Chromatography
    • CAMAG system
    • Electro-UltraFiltration System
    • UHPLC System - PDA & PDA/QDA

Modification date : 14 December 2023 | Publication date : 23 June 2023 | Redactor : Com INRAE HdF