Thesis: Identification of relevant variables

Thesis: Identification of relevant variables to assess the genetic variability of photosynthetic resilience in pea during cold acclimation

During our research on pea frost tolerance, results from "omics" approaches point to genes involved in photosynthetic activity during cold acclimation. To pursue genetic and breeding approaches, we need to identify precise and objective phenotyping variables associated with these mechanisms.

In the first part of the thesis, pea genotypes with contrasting cold acclimation and frost tolerance capabilities will be characterized using parameters derived from chlorophyll fluorescence imaging and hyperspectral imaging (reflectance).
The second part of the thesis aims to place the results of the first part in an environmental context representative of varietal selection in the field. Relay variables (fluorescence point, electrolyte leakage measurements) will be evaluated under controlled conditions and in the field to study correlations with frost tolerance on the one hand, and with imaging variables on the other.
All these data will be used to test the hypothesis of a sufficient differential, in the field, between tolerant and sensitive varieties for the fluorescence and reflectance variables most closely correlated with frost tolerance.

Required profile: The candidate should have a basic background in plant biology. He/she should be comfortable with the use of algorithms in the context of their learning mechanisms. Initial experience in plant experimentation would be an asset: design of experimental plans, observations and measurements, data processing and statistical analysis (R language).

 

Modification date : 21 March 2024 | Publication date : 21 March 2024 | Redactor : Com INRAE HdF